Flow Cytometry

The Becton Dickinson Biosciences FACSCalibur  is a four color flow cytometer that can maximize the data collected from small samples.  Others applications involve the analysis of bacteria, DNA, stem cells, leukemia samples, cytokines and BD cytometric bead arrays. The unit can perform analysis from a single tube, not from an auto sampler or 96 well format.  Sorting is not available on this unit.  DNA analysis software as well as FlowJo and Cell Quest Pro are available.  The Mac computer is equipped with a USB flash drive, zip drive and a CD burner  for saving and transporting data.  This instrument is located at the Waisman Center in room T635B. 

• Online sign-up calendar 

• Usage Fees

• All individuals using this flow cytometer must have user accounts set up prior to sample analysis.  To set up a user account for this instrument contact Pam Westmark.  All users must fill out a biosafety form and a billing information form prior to use.

  • Bio-Safety Form  (MS Word)

  • Billing Information Form (MS Word)

• For more information on the BD FACSCalibur and its applications use the following links: 

  • For technical assistance or technician analyzed samples contact Pam Westmark.

  • For FACS cell sorting or other applications not offered here contact the UWCCC Flow Cytometry facility.  Preparing cells for analysis on a BD Biosciences  FASCalibur Flow Cytometer.

Last Updated: June 26, 2008