Fluorescence Microscopy

The principle of fluorescence microscopy is to excite your tissue at one wavelength and measure emission at a different wavelength. For example, we often use blue light to excite fluorophores such as FITC and measure green light emission. A very basic explanation can be found on Wikipedia.

Our Zeiss photomicroscope has four different filter cubes for fluorescence microscopy, allowing users to capture up to four channels for the same field of view. Our system is optimized for imaging DAPI, FITC, Rhodamine and Cy5, so if your fluorophore has properties similar to these molecules, we should be able to image it using our setup.

Related resources can be found here.