Azure c600 Phosphorimaging System

  • Image Resolution: 8.3 MP cooled CCD camera (60 or 120 micron resolution)
  • Lens: 25mm/F0.95motorized
  • Maximum Field of View: 20 cm x 15 cm
  • Image output: 16-bit tiff or jpeg

Illumination specifications:

Excitation Emission
Illumination  Light Mode Excitation Wavelength Filter Name Emission wavelength Applications
Trans UV UV Transilluminator 302 nm c-Orange 595 nm ± 55 nm Coomassie Blue, SYPRO Orange, SYBR Safe, SYBR Green, GFP, AF488
365 nm
EPI Blue LED-Blue 470 nm
EPI RGB LED-Blue 457 ± 10 nm c-Blue 497 ± 16 nm “Green” Cy2, GFP, FITC, AF 488
LED-Green 530 ± 10 nm c-Green 576 ± 15 nm “Red” Cy3, dsRed, RFP, AF547
LED-Red 628 ± 10 nm c-Red 685 ± 25 nm “Far Red” Cy-5, AF633, AF647
EPI NIR Laser 660 ± 3 nm c-NIR700 720 ± 20 nm IR 680, Licor 700, AF680
Laser 785 ± 3 nm c-NIR800 831 ± 35 nm IR 800, Licor 800,

Imaging modalities based on light source selection:

UV 302: For dyes that are UV excitable (No EtBr please)

UV 365: For cutting bands out of gel

EPI Blue: For dyes excitable in the “blue light” range (SYBR green)

Visible: For dyes that do not require additional excitation- will require Orange Tray

ChemiLuminescence: will require chemitray

RGB: For fluorescent Western blots labeled with (“Far Red”-Cy5/”Red”-Cy3/”Green”-Cy2 or dyes with similar excitation and emission spectrums: will require chemitray

Near Infrared (NIR): For Western blots labeled with NIR dyes: will require chemitray

Custom: You can modify the focus, exposure, light source, and use these in combination to create your own custom capture settings.