Azure c600 Phosphorimaging System

• Image Resolution: 8.3 MP cooled CCD camera (60 or 120 micron resolution)
• Lens: 25mm/F0.95motorized
• Maximum Field of View: 20 cm x 15 cm
• Image output: 16-bit tiff or jpeg

Illumination specifications:

Excitation			Emission
Illumination	Light Mode	Excitation Wavelength	Filter Name	Emission wavelength	Applications
Trans UV	UV Transilluminator	302 nm	c-Orange	595 nm ± 55 nm	Coomassie Blue, SYPRO Orange, SYBR Safe, SYBR Green, GFP, AF488
		365 nm
EPI Blue	LED-Blue	470 nm
EPI RGB	LED-Blue	457 ± 10 nm	c-Blue	497 ± 16 nm	“Green” Cy2, GFP, FITC, AF 488
	LED-Green	530 ± 10 nm	c-Green	576 ± 15 nm	“Red” Cy3, dsRed, RFP, AF547
	LED-Red	628 ± 10 nm	c-Red	685 ± 25 nm	“Far Red” Cy-5, AF633, AF647
EPI NIR	Laser	660 ± 3 nm	c-NIR700	720 ± 20 nm	IR 680, Licor 700, AF680
	Laser	785 ± 3 nm	c-NIR800	831 ± 35 nm	IR 800, Licor 800,

Imaging modalities based on light source selection:

UV 302: For dyes that are UV excitable (No EtBr please)

UV 365: For cutting bands out of gel

EPI Blue: For dyes excitable in the “blue light” range (SYBR green)

Visible: For dyes that do not require additional excitation- will require Orange Tray

ChemiLuminescence: will require chemitray

RGB: For fluorescent Western blots labeled with (“Far Red”-Cy5/”Red”-Cy3/”Green”-Cy2 or dyes with similar excitation and emission spectrums: will require chemitray

Near Infrared (NIR): For Western blots labeled with NIR dyes: will require chemitray

Custom: You can modify the focus, exposure, light source, and use these in combination to create your own custom capture settings.