- Contact CMN staff to be trained on this equipment or if you have questions about using this microscope.
- Rates
- Online Calendar
Microscopy
Leica Microscope platform
- Fully motorized Upright stereomicroscope focus drive with 150 mm travel range
- Z6 APO A motorized zoom and X-Y stage
- Super Z-galvo stage capable of 10 nm Z-steps (500 um range)
- Stage adaptors for slides, 35 mm TC dishes, multiwell plates
- Infinity corrected objectives (include table)
- Laser Safety Chamber
- Transmitted light intensity control
- Monochrome CCD camera (Leica DC365 FX)
- Variable NA objectives (include table)
| Magnification | Immersion Media | Working Distance (mm)* |
| 1x | None | 97 |
| 2x | None | 39 |
| 5x | None | 19 |
- Epifluorescence filter sets
| Filter Set | Excitation | Dichroic | Emission |
| UV-Blue (Leica D) | 355-425 nm | 455 | LP 470 |
| Blue-Green (Leica I3) | 450-490 | 510 | LP 515 |
| Green-Red (Leica N2.1) | 515-560 | 580 | LP 590 |
| Red-Far Red (Leica Y5) | 620/60 | 660 | 700/75 |
Leica Macroconfocal
- 1 spectral PMT fully tunable between 430-750 nm
- Transmitted light detector
- Acousto Optical Tunable Filter (AOTF)
- Lasers and recommended fluorophores (include table)
| channel | Laser | Color (pseudocolor) | Recommended Fluorophores
(AF=alexafluor) |
| 1 | 405 Diode
25 mw |
Blue (blue) | DAPI, Hoechst, AF 405, Pacific Blue, Cascade Blue, Coumarin |
| 2 | 488 Diode
10 mW |
Green (green) | 488: AF488, FITC, eGFP, Cy2, YO-Pro
|
| 3 | 561 Diode
20 mw |
Red (red) | AFs 546, 551, 568, Cy3, DiI, DsRed, mCherry, PE, PI, RFP, tdTomato, TRITC |
| 4 | 635 Diode
10 mw |
Far Red (white) | AF 633, 647, Cy5, To-PRO-3 |