Nikon A1R-HD Laser Scanning Confocal Microscope

Infinity Corrected Lenses:

Objective Magnification Perfect Focus compatible Numerical Aperature (NA) Immersion Media Working Distance (mm) Objective Type Primary Use
10x 0.45 NA-Air 4 Plan Apo Fixed Samples
20x PF 0.75 NA-Air 1 Plan Apo Fixed or Live
20x 0.75 Multi (water, oil, glycerin) 0.35 Plan Fluor Live Samples
60x 1.2 Water 0.27 (0.13-0.19 mm) Plan Apo Live Samples
60x PF 1.4 Oil 0.13 Plan Apo Fixed Samples
100x 1.4 Oil 0.17 Plan Apo Fixed Samples

 

Epifluorescent filters for sample visualization (no camera)

 

Filter Set Excitation Dichroic Emission (bandpass)
DAPI HC HiSN Zero Shift 325-375 nm 400 435-485
GFP HC HiSN Zero Shift 450-490 495 500-550
TxRED HC HiSN Zero Shift 540-580 585 593-668

 

CONFOCAL PLATFORM (Nikon A1R-HD, 2018):

4 diode Lasers (405 nm, 488nm, 561 nm, 640 nm)

4 PMTS (Standard: Blue and Far Red, GaASP: Green and Red)

32 channel spectral detector

Transmitted light Detector for Differential Interference Contrast (DIC) microscopy

NIS Elements Advanced software v 5.2

Galvonometric scanner: Max Pixel size 4096×4096, 512×512 scan speed 4 fps, zoom: 1-1000 variable

Resonant scanner: max. 1024×1024 pixels, 30 fps (512 x 512 pixels) to 230 fps
(512 x 64 pixels), 15,600 lines/sec (line speed). Zoom: 7 steps (1x, 1.5x, 2x, 3x, 4x, 6x, 8x)
Pinhole: Variable

Spectral Detector: image acquisition speed: 4 fps (256×256), 1000 lps; 2.5nm, 6nm or 10 nm wavelength range

Z step 0.025 um

Lasers and recommended fluorophores:

channel Laser Color (pseudocolor) PMT/Detector (virtual filter, BP nm) Recommended Fluorophores
(AF=alexafluor)
1 405 Diode

100 mw Coherent

Blue (blue) 1 (450/50 480DCXR) DAPI, Hoechst, AF 405, Pacific Blue, Cascade Blue, Coumarin
2 488 Diode

 

Green (green) 2 (485/40 520 DCXR

 

488: AF488, FITC, eGFP, Cy2, YO-Pro

 

3 561 Diode

25 mw Coherent

Red  (red) 3 (600/50 640 DXCR) AFs 546, 551, 568, Cy3, DiI, DsRed, mCherry, PE, PI, RFP, tdTomato, TRITC
4 640 Diode

100 mw Coherent

Far Red (pink) 4 (650 LP 3FW) AF 633, 647, Cy5, To-PRO-3