Leica TCS-LSI Macroconfocal

Leica Microscope platform

  • Fully motorized Upright stereomicroscope focus drive with 150 mm travel range
  • Z6 APO A motorized zoom and X-Y stage
  • Super Z-galvo stage capable of 10 nm Z-steps (500 um range)
  • Stage adaptors for slides, 35 mm TC dishes, multiwell plates
  • Infinity corrected objectives (include table)
  • Laser Safety Chamber
  • Transmitted light intensity control
  • Monochrome CCD camera (Leica DC365 FX)
  • Variable NA objectives (include table)
Magnification Immersion Media Working Distance (mm)*
1x None 97
2x None 39
5x None 19
  • Epifluorescence filter sets

 

Filter Set Excitation Dichroic Emission
UV-Blue (Leica D) 355-425 nm 455 LP 470
Blue-Green (Leica I3) 450-490 510 LP 515
Green-Red (Leica N2.1) 515-560 580 LP 590
Red-Far Red (Leica Y5) 620/60 660 700/75

 

Leica Macroconfocal

  • 1 spectral PMT fully tunable between 430-750 nm
  • Transmitted light detector
  • Acousto Optical Tunable Filter (AOTF)
  • Lasers and recommended fluorophores (include table)
channel Laser Color (pseudocolor) Recommended Fluorophores

(AF=alexafluor)

1 405 Diode

25 mw

Blue (blue) DAPI, Hoechst, AF 405, Pacific Blue, Cascade Blue, Coumarin
2 488 Diode

10 mW

Green (green) 488: AF488, FITC, eGFP, Cy2, YO-Pro

 

3 561 Diode

20 mw

Red (red) AFs 546, 551, 568, Cy3, DiI, DsRed, mCherry, PE, PI, RFP, tdTomato, TRITC
4 635 Diode

10 mw

Far Red (white) AF 633, 647, Cy5, To-PRO-3