Nikon C1 Upright Confocal Microscope

Nikon C1 Microscope Platform

  • Nikon E600 Upright Microscope with Z focus control (mechanical stage)
  • Infinity corrected Nikon lenses (*using a 0.17mm coverglass):
Mag/Na Immersion Media Working Distance (mm)* Objective Type
10x/0.45 none 4.0 Plan Apo
40x/1.30 Oil 0.2 Plan Fluor
60x/1.40 Oil 0.21 Plan Apo
100x/1.40 Oil 0.17 Plan Apo

Epifluorescence filter sets:

Filter Set Excitation Dichroic Emission (bandpass)
DAPI/FITC double 400-418 nm DAPI

478-495 nm FITC

409 (DAPI)

487 (FITC)

435-470 (DAPI)

505-570 (FITC)

FITC-HYQ 460-500 505 510-560
TRITC-HYQ 530-560 565 590-650

Nikon C1 Laser Scanning Confocal

  • Recommended Fluors
Channel Laser PMT/Detector (virtual filter, BP nm) Recommended Fluorophores
1 (Blue) 405 Diode

100 mw Coherent

1 (450/40) DAPI, Hoechst, AlexaFluor 405, Pacific Blue, Cascade Blue, Coumarin
2 (green) 488 Multiline

50mw Argon Gas

 

 

3 (525/50)

458: eCFP, BO-Pro-1, Alexafluor 430

477: eCFP

488: Alexa488, FITC, eGFP, Cy2, YO-Pro

514: Alexa514, YFP

3 (red) 543 Diode

1mw Melles Griot

2 (595/50) Alexa 546, 551, 568, 594, Cy3, DiI, DsRed, mCherry, PE, PI, RFP, tdTomato, TRITC
4 (far red/pink) 640 Diode

40 mw Coherent

1 (660 LP) Alexa 633, 647, Cy5, To-PRO-3