Zeiss Photomicroscope

Zeiss Photomicroscope Platform

Photomicroscope has two color digital cameras suitable for collecting publication quality epifluorescence and brightfield images or performing stereology.

High NA Objectives

Mag/Na Immersion Media Working Distance (mm)* Objective Type
2.5x/0.075 None-dry 9.5 Plan neo-FLUAR
10x/0.30 None-dry 5.2 Plan neo-FLUAR
20x/0.50 None-dry 2.0 Plan neo-FLUAR
40x/1..30 Oil 0.21 Plan neo-FLUAR
100x/1.30 Oil 0.20 Plan neo-FLUAR

 

This instrument platform includes a high precision motorized stage and optics that are optimized for brightfield and fluorescence stereological analysis. DIC or phase-contrast optics are NOT available on this system. AxioVision software is available for widefield image collection.

Fluorescence Microscopy

Fluorescence microscopy involves the excitation of fluorophores at a specific wavelength and the measurement of emitted light at another wavelength. The Zeiss photomicroscope has the following fluorescent filters

Filter Set Excitation Dichroic Emission (bandpass)
DAPI (Zeiss49) 365/50 395 445/50
FITC (Zeiss 38HE) 470/40 495 525/50
Rhodamine (Chroma CZ 915 546/10 565 605/55
Cy5 (Zeiss 50) 640/30 660 690/50

Brightfield Microscopy

Brightfield microscopy is used to examine cells and tissues that can be visualized without fluorescence, e.g H&E, cresyl violet, DAB.

Example of H&E Stain
Example of H&E Stain
 Example of Cresyl Violet Stain
Example of Cresyl Violet Stain

Stereology

Stereology is a method that allows the unbiased sampling of tissues for accurate counts or measurements of structures within tissue sections. The CMN Core has a Zeiss photomicroscope equipped with a high resolution digital camera, a high precision motorized stage, and a computer with the Stereo Investigator software package (MicroBrightfield Inc) for investigators interested in performing stereologic analyses in their studies. Examples of common uses of stereology include performing cell counts, measuring the lengths of blood vessels, axons or other linear structures within tissues, and estimating volumes of cells, lesions, infarcts or other structures.