PhD, University of Pennsylvania School of Medicine
Associate Director, IDDRC
Faculty Co-Director, Rodent Models Core
Associate Professor of Genetics & Neurology
Our long-term goal is to understand the molecular mechanism underlying DNA methylation-dependent epigenetic regulation of brain functions. Our current focus is on the central role of MeCP2 (methyl-CpG binding protein 2), a molecular linker between DNA methylation and chromatin remodeling and transcriptional control, in the development and function of the nervous system. The functional significance of such a molecular linker is highlighted by the fact that mutations in the MECP2 gene cause Rett syndrome (RTT), a debilitating neurodevelopmental disorder that shares many features with autism. Naturally, our studies include basic research aimed at understanding the function of MeCP2 and translational research aimed at understanding disease pathology and developing effective treatment. These two types of research are tightly interwoven, because on one hand, the need to solve a practical problem in translational research will always influence the direction of basic research; and on the other hand, fundamental mechanisms revealed by basic research will ultimately guide the effort in treating/curing the disease. Below are some highlights of our effort on both fronts.
In general, we use genetic engineering in mouse embryonic stem (mES) cells to manipulate the function of Mecp2 in vivo, and integrate analyses at the molecular, cellular, electrophysiological, animal behavioral, and genomic levels to study these genetically engineered mice.
In vivo function of neuronal activity-induced MeCP2 phosphorylation
To understand what functional role DNA methylation-dependent epigenetic mechanisms may play in the development, maintenance and function of the mammalian nervous system, it is important to study how the recognition of DNA methylation and the interpretation of this epigenetic information by MeCP2 may be dynamically regulated in neuronal nuclei in response to extracellular signals. Several lines of evidence suggest that neuronal activity-induced phosphorylation (NAIP) of MeCP2 may work as a molecular switch on the chromatin to integrate the extrinsic signals with intrinsic transcriptional programs and generate adaptive outputs. Yet it was not clear what, if any, function requires such phosphorylation in the in vivo brain. We generated the Mecp2S421A;S424A knockin mice to abolish NAIP of MeCP2, and provided the first genetic evidence that NAIP of MeCP2 is involved in regulating several important functions in the in vivo brain, including promoter occupancy, gene transcription, excitatory synaptogenesis, long-term potentiation, and learning and memory (Li et al, 2011).
MeCP2 phosphorylation underlies synaptic scaling
Synaptic scaling is a form of non-Hebbian homeostatic plasticity that allows neurons to regulate overall excitability in response to changes in network neuronal activity levels. While it is known that neuronal activity can induce phosphorylation of MeCP2 and that MeCP2 can regulate synaptic scaling, the molecular link between MeCP2 phosphorylation and synaptic scaling remains undefined. Recent work in my lab demonstrated that MeCP2 phosphorylation is required for bicucullin-induced synaptic scaling down in mouse hippocampal neurons, and that this phenotype is mediated by mGluR5. These findings reveal the first downstream effector protein linking the nuclear function of MeCP2 phosphorylation with neurotransmiter receptors at the synapse, thus advancing our understanding of the role of MeCP2 in synaptic scaling at the molecular level (Zhong et al, 2012).
A novel role of MeCP2 phosphorylation in regulating adult neurogenesis
We discovered that S421 is also phosphorylated in adult neural progenitor cells (aNPC) isolated from the hippocampus. Interestingly, the stimulus, the regulation and the function of S421 phosphorylation in aNPCs are completely different from those in post-mitotic neurons. In aNPCs, MeCP2 S421 phosphorylation is induced by growth factors, linked to cell cycle, directly regulated by aurora kinase B, and plays critical roles in regulating the proliferation and differentiation of aNPCs through the Notch signaling pathway. These new findings further generalize MeCP2 phosphorylation as a common regulatory module in cellular functions. (Li et al, 2014).
Together with our unpublished results, these findings raise the possibility that dynamic phosphorylation states at multiple sites on MeCP2 may form a combinatorial code, presenting another epigenetic regulatory module in addition to DNA methylation and histone codes. To directly test this hypothesis, we are in the process of generating several novel Mecp2 knockin alleles carrying combinations of point mutations that either abolish or mimic phosphorylation at S80 and S421, and study the functional output of each combinatorial code in the well-established paradigm of adult neurogenesis. In addition, we plan to combine our mouse genetics expertise with cutting edge genomic and proteomic technologies to reveal the genome wide distribution of MeCP2 and its interacting partners, and how such distributions and interactions are regulated by stimulus-induced post-translational modification of MeCP2.
We have used the Mecp2 knockout mice to test the therapeutic potential of candidate drugs. Recently, to complement our in vivo mouse models, we have generated congenic pairs of iPSC lines from several female RTT patients carrying either common or rare RTT mutations, differentiated both the mutant and wild type iPSC lines into neurons and astrocytes, and observed characteristic RTT phenotypes in these cells.
Studying disease mechanisms and testing treatment in RTT mouse models
Following up our earlier discovery that BDNF (brain-derived neurotrophic factor) is a key modulator of disease progression in a RTT mouse model (Chang et al, 2006), we evaluated the therapeutic efficacy of 7,8-dihydroxyflavone (a selective agonist of TrkB, the high affinity BDNF receptor) in a RTT mouse model, and showed that it could alleviate several hallmark RTT symptoms (Johnson et al, 2012). These results suggest that 7,8-dihydroxyflavone have therapeutic a potential for treating RTT.
Establishing RTT iPSC-derived human neurons and astrocytes as in vitro models and platforms for future drug screen
Because of potential differences in the underlying biology between humans and common research animals, there is a need to establish cell culture-based human models for studying disease mechanisms to validate and expand the knowledge acquired in animal models. Taking advantage of the nonrandom pattern of X chromosome inactivation in female induced pluripotent stem cells (iPSC), we have generated congenic pairs of wild type and mutant iPSC lines from several female RTT patients with common and rare RTT mutations. R294X (arginine 294 to stop codon) is a common mutation carried by 5-6% of RTT patients. We differentiated three R294X iPSC lines and their congenic wild type control iPSC into neurons with high efficiency and consistency, and observed characteristic RTT pathology in R294X neurons. These congenic iPSC lines provide unique resources to the RTT research community for studying disease pathology, screening for novel drugs, and testing toxicology (Ananiev et al, 2011).
Most recently, we have differentiated three congenic pairs (V247X, R294X and R306C) of RTT iPSC lines into highly pure astrocytes, and studied the glial contribution to RTT pathology. Our data showed that mutant astrocytes and the medium conditioned by mutant astrocytes had adverse effect on the growth and morphology of wild type neurons. These results fully validate previous finding in RTT mouse models, and further establish human RTT iPSCs and its derivatives as valid in vitro disease models for studying pathogenesis and screening for drugs (Williams et al, 2014).We are using the astrocytes differentiated from congenic RTT iPSC lines to study cell autonomous phenotypes and the underlying mechanisms in RTT astrocytes, and develop this platform for future drug screens. Once we identify candidate drugs using our in vitro platform, we will validate the top ranked ones in our RTT mouse models in vivo. Results from these studies will ultimately help finding an effective treatment/cure for RTT.
Gao Y, Su J, Guo W, Polich ED, Magyar DP, Xing Y, Li H, Smrt RD, Chang Q, Zhao X. (2015) Inhibition of miR-15a Promotes BDNF Expression and Rescues Dendritic Maturation Deficits in MeCP2-Deficient Neurons. Stem Cells. 33(5):1618-29. doi: 10.1002/stem.1950.
Musah S, Wrighton PJ, Zaltsman Y, Zhong X, Zorn S, Parlato MB, Hsiao C, Palecek SP, Chang Q, Murphy WL, Kiessling LL. (2014) Substratum-induced differentiation of human pluripotent stem cells reveals the coactivator YAP is a potent regulator of neuronal specification. Proceedings of the National Academy of Sciences USA. 23;111(38):13805-10. doi: 10.1073/pnas.1415330111.
Williams EC, Zhong X, Mohamed A, Li R, Liu Y, Dong Q, Ananiev GE, Mok JC, Lin BR, Lu J, Chiao C, Cherney R, Li H, Zhang SC, Chang Q. (2014) Mutant astrocytes differentiated from Rett syndrome patients-specific iPSCs have adverse effects on wild-type neurons. Human Molecular Genetics. 23(11):2968-80.
Guo JU, Su Y, Shin JH, Shin J, Li H, Xie B, Zhong C, Hu S, Le T, Fan G, Zhu H, Chang Q, Gao Y, Ming GL, Song H. (2014) Distribution, recognition and regulation of non-CpG methylation in the adult mammalian brain. Nature Neuroscience. 17(2):215-22.
Williams EC, Zhong X, Mohamed A, Li R, Liu Y, Dong Q, Ananiev GE, Mok JC, Lin BR, Lu J, Chiao C, Cherney R, Li H, Zhang SC, Chang Q. (2014) Mutant astrocytes differentiated from Rett syndrome patients-specific iPSCs have adverse effects on wild-type neurons. Human Molecular Genetics. 1;23(11):2968-80.
Li H, Zhong X, Chau KF, Santistevan NJ, Guo W, Kong G, Li X, Kadakia M, Masliah J, Chi J, Jin P, Zhang J, Zhao X, Chang Q. (2014) Cell cycle-linked MeCP2 phosphorylation modulates adult neurogenesis involving the Notch signaling pathway. Nature Communications. 25;5:5601.
Zhong X, Li H, and Chang Q. (2012) MeCP2 phosphorylation is required for modulating synaptic scaling through mGluR5. Journal of Neuroscience. 12;32(37):12841-7.
Ananiev G, Williams EC, Li H, Chang Q. (2011) Isogenic Pairs of Wild Type and Mutant Induced Pluripotent Stem Cell (iPSC) Lines from Rett Syndrome Patients as In Vitro Disease Model. PloS ONE. 6(9):e25255.
Li H, Zhong X, Chau KF, Williams EC, Chang Q. (2011) Loss of Activity-Induced Phosphorylation of MeCP2 Enhances Synaptogenesis, LTP, and Spatial Memory. Nature Neuroscience. 14(8):1001-8.
Chang Q, Khare GD, Dani VS, Nelson SB, and Jaenisch, R. (2006) The disease progression of Mecp2 mutant mice is affected by the level of BDNF expression. Neuron. 49(3):341-348.
Dani VS, Chang Q, Maffei A, Turrigiano GG, Jaenisch R, Nelson SB. (2005) Reduced cortical activity due to a shift in the balance between excitation and inhibition in a mouse model of Rett syndrome. Proceedings of the National Academy of Sciences of the United States of America. 30;102(35):12560-5.
Chen WG, Chang Q, Lin Y, Meissner A, West AE, Griffith EC, Jaenisch R, Greenberg ME. (2003) Derepression of BDNF transcription involves calcium-dependent phosphorylation of MeCP2. Science. 31;302(5646):885-9.