Krishanu Saha, PhD

Position title: Associate Professor, Biomedical Engineering

PhD, University of California-Berkeley

Contact Information

Wisconsin Institute for Discovery
330 North Orchard Street
Room 4164
Madison, WI 53715
608.316.4313
ksaha@wisc.edu
Saha Lab

Research Statement

Cellular reprogramming allows for the generation of a self-renewing and patient-specific cell source for drug discovery, cell therapies, and regenerative medicine through the generation of human induced pluripotent stem cells (hiPSCs). hiPSCs can grow indefinitely in culture and retain the ability to mature into any cell type of the human body.

By bringing together stem cell biology, genome engineering and biomaterials expertise, the Saha lab generates new tools for use with hiPSCs to ask unique questions about human biology and disease.

Selected Publications

Steyer B, Bu Q, Cory E, Jiang K, Duong S, Sinha D, Steltzer S, Gamm D, Chang Q, Saha K. (2018). Scarless Genome Editing of Human Pluripotent Stem Cells via Transient Puromycin Selection. Stem Cell Reports, 13;10(2):642-654. doi: 10.1016/j.stemcr.2017.12.004.
Movaghar A, Mailick MR, Sterling A, Greenberg J, Saha K. (2017) Automated Screening for Fragile X Premutation Carriers Based on Linguistic and Cognitive Computational Phenotypes. Scientific Reports . 7(1):2674. doi: 10.1038/s41598-017-02682-4. PMCID:PMC5454004.
Li M, Zhao H, Ananiev GE, Musser MT, Ness KH, Maglaque DL, Saha K, Bhattacharyya A, Zhao X. (2017). Establishment of Reporter Lines for Detecting Fragile X Mental Retardation (FMR1) Gene Reactivation in Human Neural Cells. Stem Cells, 35(1):158-169. doi: 10.1002/stem.2463.
Steyer B, Carlson-Stevermer J, Angenent-Mari N, Khalil A, Harkness T, Saha K. (2016) High content analysis platform for optimization of lipid mediated CRISPR-Cas9 delivery strategies in human cells. Acta Biomaterialia, 34:143-158. doi: 10.1016/j.actbio.2015.12.036.
Carlson-Stevermer J, Goedland M, Steyer B, Movaghar A, Lou M, Kohlenberg L, Prestil R, Saha K. (2016) High-Content Analysis of CRISPR-Cas9 Gene-Edited Human Embryonic Stem Cells. Stem Cell Reports. 12;6(1):109-20. doi: 10.1016/j.stemcr.2015.11.014.
Laperle A, Hsiao C, Lampe M, Mortier J, Saha K, Palecek SP, Masters KS. (2015) α-5 Laminin Synthesized by Human Pluripotent Stem Cells Promotes Self-Renewal. Stem Cell Reports. 11;5(2):195-206. doi: 10.1016/j.stemcr.2015.06.009.
Harkness T, McNulty JD, Prestil R, Seymour SK, Klann T, Murrell M, Ashton RS, Saha K. (2015) High-content imaging with micropatterned multiwell plates reveals influence of cell geometry and cytoskeleton on chromatin dynamics. Biotechnology Journal. 10(10):1555-67. doi: 10.1002/biot.201400756.