Donna Werling, PhD

Position title: Assistant Professor, Genetics

PhD, University of California, Los Angeles

Contact Information

Laboratory of Genetics
5262 Genetics and Biotechnology Center
Madison, WI 53706-1580
(608) 262-3113
dwerling@wisc.edu
Werling Lab

Research Statement

Donna Werling is interested in characterizing sex-differential risk mechanisms in autism spectrum disorder (ASD). During her doctoral work in the laboratory of Dan Geschwind at the University of California, Los Angeles, Werling used functional genomics, human genetics and bioinformatics approaches to understand the relationship between sex and genetic risk in ASD.

During postdoctoral work in the laboratories of Matt State and Stephan Sanders at the University of California, San Francisco, she continued working on mechanisms underlying sex differences in ASD, while also expanding her expertise on ASD genetic risk through her collaborative efforts to develop the Category-Wide Associate Study (CWAS), an analytical framework for association testing of functional defined categories of coding and noncoding de novo variants.

Werling will utilize the expertise gained over the course of her Ph.D. and postdoctoral training to characterize sex differences at both the cellular and tissue level in both ASD mouse models and individuals with ASD to understand the mechanisms underlying male bias in ASD prevalence.

Selected Publications

PubMed

Kim, S. W., Lee, H., Song, D. Y., Lee, G. H., Ji, J., Park, J. W., Han, J. H., Lee, J. W., Byun, H. J., Son, J. H., Kim, Y. R., Lee, Y., Kim, J., Jung, A., Lee, J., Kim, E., Kim, S. H., Lee, J. H., Satterstrom, F. K., Girirajan, S., … An, J. Y. (2024). Whole genome sequencing analysis identifies sex differences of familial pattern contributing to phenotypic diversity in autism. Genome medicine, 16(1), 114. https://doi.org/10.1186/s13073-024-01385-6
Singh, A. P., Fromandi, M., Pimentel-Alarcón, D., Werling, D. M., Gasch, A. P., & Yu, J. J. (2024). Intrinsic Gene Expression Correlates of the Biophysically Modeled Diffusion Magnetic Resonance Imaging Signal. Biological psychiatry global open science, 5(2), 100430. https://doi.org/10.1016/j.bpsgos.2024.100430
Kissel, L. T., Pochareddy, S., An, J. Y., Sestan, N., Sanders, S. J., Wang, X., & Werling, D. M. (2024). Sex-Differential Gene Expression in Developing Human Cortex and Its Intersection With Autism Risk Pathways. Biological psychiatry global open science, 4(4), 100321. https://doi.org/10.1016/j.bpsgos.2024.100321
Kim, Y., Jeong, M., Koh, I. G., Kim, C., Lee, H., Kim, J. H., Yurko, R., Kim, I. B., Park, J., Werling, D. M., Sanders, S. J., & An, J. Y. (2024). CWAS-Plus: Estimating category-wide association of rare noncoding variation from whole-genome sequencing data with cell-type-specific functional data. medRxiv : the preprint server for health sciences, 2024.04.15.24305828. https://doi.org/10.1101/2024.04.15.24305828
Shen, M., Sirois, C. L., Guo, Y., Li, M., Dong, Q., Méndez-Albelo, N. M., Gao, Y., Khullar, S., Kissel, L., Sandoval, S. O., Wolkoff, N. E., Huang, S. X., Xu, Z., Bryan, J. E., Contractor, A. M., Korabelnikov, T., Glass, I. A., Doherty, D., Birth Defects Research Laboratory, Levine, J. E., … Zhao, X. (2023). Species-specific FMRP regulation of RACK1 is critical for prenatal cortical development. Neuron, 111(24), 3988–4005.e11. https://doi.org/10.1016/j.neuron.2023.09.014
Kissel, L. T., & Werling, D. M. (2022). Neural Transcriptomic Analysis of Sex Differences in Autism Spectrum Disorder: Current Insights and Future Directions. Biological Psychiatry, 91(1), 53–60. https://doi.org/10.1016/j.biopsych.2020.11.023
Zhang, Y., Lu, Q., Ye, Y., Huang, K., Liu, W., Wu, Y., Zhong, X., Li, B., Yu, Z., Travers, B. G., Werling, D. M., Li, J. J., & Zhao, H. (2021). SUPERGNOVA: local genetic correlation analysis reveals heterogeneous etiologic sharing of complex traits. Genome biology, 22(1), 262. https://doi.org/10.1186/s13059-021-02478-w
Liang, L., Fazel Darbandi, S., Pochareddy, S., Gulden, F. O., Gilson, M. C., Sheppard, B. K., Sahagun, A., An, J. Y., Werling, D. M., Rubenstein, J. L. R., Sestan, N., Bender, K. J., & Sanders, S. J. (2021). Developmental dynamics of voltage-gated sodium channel isoform expression in the human and mouse brain. Genome medicine, 13(1), 135. https://doi.org/10.1186/s13073-021-00949-0
Werling DM, Pochareddy S, Choi J, An JY, Sheppard B, Peng M, Li Z, Dastmalchi C, Santpere G, Sousa AMM, et al. (2020). Whole-Genome and RNA Sequencing Reveal Variation and Transcriptomic Coordination in the Developing Human Prefrontal Cortex. Cell Reports, 31(1):107489. doi: 10.1016/j.celrep.2020.03.053. PMID: 32268104
Werling DM. (2019). Clinically Defined Subtypes of Bipolar Disorder Are Reflected in Genomic Architecture. Biological Psychiatry, 86(2):78-80. doi: 10.1016/j.biopsych.2019.05.010.
Li M, Santpere G, Imamura Kawasawa Y, Evgrafov OV, Gulden FO, Pochareddy S, Sunkin SM, Li Z, Shin Y, Zhu Y, Sousa AMM, Werling DM, Kitchen RR, Kang HJ, Pletikos M, Choi J, Muchnik S, Xu X, Wang D, Lorente-Galdos B, Liu S, Giusti-Rodríguez P, Won H, de Leeuw CA, Pardiñas AF; BrainSpan Consortium; PsychENCODE Consortium; PsychENCODE Developmental Subgroup, Hu M, Jin F, Li Y, Owen MJ, O’Donovan MC, Walters JTR, Posthuma D, Reimers MA, Levitt P, Weinberger DR, Hyde TM, Kleinman JE, Geschwind DH, Hawrylycz MJ, State MW, Sanders SJ, Sullivan PF, Gerstein MB, Lein ES, Knowles JA, Sestan N. (2018). Integrative functional genomic analysis of human brain development and neuropsychiatric risks. Science, 362(6420). pii: eaat7615. doi: 10.1126/science.aat7615.
An JY, Lin K, Zhu L, Werling DM, Dong S, Brand H, Wang HZ, Zhao X, Schwartz GB, Collins RL, et al. (2018). Genome-wide de novo risk score implicates promoter variation in autism spectrum disorder. Science, 14;362(6420). pii: eaat6576. doi: 10.1126/science.aat6576.
Werling DM, Brand H, An JY, Stone MR, Zhu L, Glessner JT, Collins RL, Dong S, Layer RM, Markenscoff-Papadimitriou E, Farrell A, et al. (2018). An analytical framework for whole-genome sequence association studies and its implications for autism spectrum disorder. Nature Genetics, 26;50(5):727-736. doi: 10.1038/s41588-018-0107-y.
Sanders SJ, Neale BM, Huang H, Werling DM, An JY, Dong S; Whole Genome Sequencing for Psychiatric Disorders (WGSPD), Abecasis G, Arguello PA, Blangero J, Boehnke M, Daly MJ, Eggan K, Geschwind DH, et al. (2017). Whole genome sequencing in psychiatric disorders: the WGSPD consortium. Nature Neuroscience, 20(12):1661-1668. doi: 10.1038/s41593-017-0017-9.
Bishop SL, Farmer C, Bal V, Robinson EB, Willsey AJ, Werling DM, Havdahl KA, Sanders SJ, Thurm A. (2017). Identification of Developmental and Behavioral Markers Associated With Genetic Abnormalities in Autism Spectrum Disorder. American Journal of Psychiatry, 174(6):576-585. doi: 10.1176/appi.ajp.2017.16101115.
Werling DM, Parikshak NN, Geschwind DH. (2016). Gene expression in human brain implicates sexually dimorphic pathways in autism spectrum disorders. Nature Communications, 7:10717. doi: 10.1038/ncomms10717.
Werling DM. (2016). The role of sex-differential biology in risk for autism spectrum disorder. Biology of Sex Differences, 16;7:58.