The UW Human Stem Cell Editing Service, located in the Waisman Center provides CRISPR-Cas9 gene editing of human pluripotent stem cells (PSCs) to campus researchers. Gene editing enables the creation of isogenic human PSC lines by correcting the gene mutation in the patient iPSCs or by knocking a mutation into otherwise normal human PSCs. The isogenic cells have the identical genetic background except for the mutation, thus providing stringent controls and avoiding much of the variability inherent in iPSCs, which will greatly enhance our ability to understand how those mutations cause particular diseases. In addition to using gene editing to correct or establish mutations in hPSCs, the service will use new technologies to enable strategies to regulate gene expression, delete genes and insert tags that allow rapid cell sorting.
The gene editing sevice provides technical services, including generation of genome edited human stem cells lines, quality control of genome edited cells, and training for lab personnel.
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