Leica Stellaris 8 Confocal

Capabilities:

• Epifluorescence light source plus monochrome camera for widefield imaging
• 405 nm diode laser plus the White light Laser (any fluorescence excitation wavelengths between 440 and 790 nm)
• HyD detectors (3 S’s and an X)
• Continuous Zoom (0.75x-48x)
• Super Galvo Z stage
• Bidirectional Galvanometric and Resonance scanheads
• Incubator for live imaging
• Objectives: 5x/0.15 NA Air, 20x/0.75 Air, 25x/0.95 water, 40x/1.1 Water, 63x/1.2 Water, 63x/1.4 oil
• Live super-resolution deconvolution mode ‘Lightning’ (pinhole adjustment plus deconvolution)
• Tausense (ability to register average photon lifetimes, Tau Contrast, Tau gating, Tau separation)
• Software Modules: Navigator/FRAP/FRET/Dye Finder

Users: please remember to use #1.5 coverslips (0.17mm)

Microscope Platform (Leica DMI8 CS, 2023)

• Fully motorized stage for optimized for tiling and navigation
• Super Galvo Z stage (500 nm movement)- especially good for live imaging
• Leica Automatic Focus Correction (AFC)
• Widefield imaging: LED 3 (LED 405, GFP, TXR), Brightfield (DIC, polarized)
• Monochrome camera ScMos 2048×2048 (6.5 um x 6.5 um pixel)
• Stage platform compatible with plates, slides, dishes

Objectives (additional optical zoom from 0.75-48x, DIC on high NA objectives):

Confocal Excitation Light Source(s):

• Near UV laser diode laser at 405nm
• White light laser — ANY wavelength from 440nm – 790nm. Up to 8 lines at once with 8nm spacing

Confocal Emission Detection (between 410nm and 850nm)

• 3 x large dynamic range Leica Detectors (HyDSs)
• 1 hybrid GaAsP/PMT (HyDX)
• Transmitted light detector with DIC polarizers for 20x, 40x, 63x objectives
• Modifiable greyscale output (8, 12, 16 bit grayscale)
• Tau-sense addition to time gates allows for software-assisted separation of dyes based on fluorescence lifetime in addition to wavelength