The Zeiss Stereology can be used to perform Fluorescence and Brightfield Microscopy, Stereology.
- Fluorescence
- Brightfield Microscopy
- Stereology
Usage
- Contact CMN staff to be trained on this equipment or if you have questions about using this microscope.
- Rates
- Online Calendar
Microscopy
Zeiss Stereology Platform
Zeiss Stereology has two color digital cameras suitable for collecting publication quality epifluorescence and brightfield images or performing stereology.
High NA Objectives
| Mag/Na | Immersion Media | Working Distance (mm)* | Objective Type |
| 2.5x/0.075 | None-dry | 9.5 | Plan neo-FLUAR |
| 10x/0.30 | None-dry | 5.2 | Plan neo-FLUAR |
| 20x/0.50 | None-dry | 2.0 | Plan neo-FLUAR |
| 40x/1..30 | Oil | 0.21 | Plan neo-FLUAR |
| 100x/1.30 | Oil | 0.20 | Plan neo-FLUAR |
This instrument platform includes a high precision motorized stage and optics that are optimized for brightfield and fluorescence stereological analysis. DIC or phase-contrast optics are NOT available on this system. AxioVision software is available for widefield image collection.
Fluorescence Microscopy
Fluorescence microscopy involves the excitation of fluorophores at a specific wavelength and the measurement of emitted light at another wavelength. The Zeiss photomicroscope has the following fluorescent filters
| Filter Set | Excitation | Dichroic | Emission (bandpass) |
| DAPI (Zeiss49) | 365/50 | 395 | 445/50 |
| FITC (Zeiss 38HE) | 470/40 | 495 | 525/50 |
| Rhodamine (Chroma CZ 915 | 546/10 | 565 | 605/55 |
| Cy5 (Zeiss 50) | 640/30 | 660 | 690/50 |
Brightfield Microscopy
Brightfield microscopy is used to examine cells and tissues that can be visualized without fluorescence, e.g H&E, cresyl violet, DAB.
 Example of H&E Stain |
 Example of Cresyl Violet Stain |
Stereology
Stereology is a method that allows the unbiased sampling of tissues for accurate counts or measurements of structures within tissue sections. The CMN Core has a Zeiss photomicroscope equipped with a high resolution digital camera, a high precision motorized stage, and a computer with the Stereo Investigator software package (MicroBrightfield Inc) for investigators interested in performing stereologic analyses in their studies. Examples of common uses of stereology include performing cell counts, measuring the lengths of blood vessels, axons or other linear structures within tissues, and estimating volumes of cells, lesions, infarcts or other structures.
- For more information about Stereology, visit stereology.info/