- Contact CMN staff to be trained on this equipment or if you have questions about using this microscope.
- Rates
- Nano HCFS Online Calendar
Microscopy
Operetta Platform
- Fully automated widefield fluorescence and brightfield imaging system
- Excitation and emission filter wheels (dichroic filter wheel matched to excitation bands)
Excitation Filter Wheel | Excitation, nm | Emission Filter wheel | Emission , nm |
UV | 360-400 | Blue | 410-480 |
Blue | 460-490 | Green | 500-550 |
Green | 520-550 | Red | 560-630 |
Red | 590-640 | ||
Far Red | 620-640 | Far Red | 650-760 |
- CCD Camera Detector (1360 x1024 pixel)
- 300 W Xenon Lamp (360-640 nm continuous spectrum)
- High resolution scanning stage (150 nm mechanical xy resolution)
- Live Cell Incubation chamber (with temperature and C02 control)
- Spinning Disk Confocal
- Laser based Auto-focus
- Many Magnification options
Magnification | Immersion Media | Working Distance (mm)* | NA | Field of View, um2 |
2x-long | None | 6.2 | 0.08 | 2.7-4 mm |
10x-long | None | 10 | 0.3 | 1351 x 1017 |
20x-long | None | 6.6-7.8 | 0.45 | 675 x 509 |
40x-long | None | 2.7-4 | 0.6 | 336 x 254 |
40x-high NA | None | 0.29 | 0.95 | 338 x 254 |
- Designed to work with microscope slides, and nearly all plastic plate forms although glass bottomed (no 1.5 coverslip) low skirt multiwall plates work best.
Operetta Software: Harmony
- Designed for multi-user and multi-department environments
- Does not require computer programming or in depth knowledge of microscopy to operate
- Easy to use database of analytical features (texture analysis, cell tracing, emission quantitation, pattern recognition)
- Ready made solutions are easy to adapt to individual user needs
- Built in data management
- Data is stored as universally compatible OME TIFF files
- It is easy to streamline data migration to Columbus Database Server
- Volocity- 3D and 4D acquisition software is available for visualization and analysis.
Columbus Scope Server
- Independent image storage, management, and high performance analysis server system
- Images from any other microscope or high content screening system can be imported into Columbus for analysis
- Statistically significant, quantitative and multi-parametric data can be generated from input images
- Users have desktop access to data and analysis