Molecular Devices Nano High Content Screening

Operetta Platform

• Fully automated widefield fluorescence and brightfield imaging system
• Excitation and emission filter wheels (dichroic filter wheel matched to excitation bands)

Excitation Filter Wheel	Excitation, nm	Emission Filter wheel	Emission , nm
UV	360-400	Blue	410-480
Blue	460-490	Green	500-550
Green	520-550	Red	560-630
		Red	590-640
Far Red	620-640	Far Red	650-760

• CCD Camera Detector (1360 x1024 pixel)
• 300 W Xenon Lamp (360-640 nm continuous spectrum)
• High resolution scanning stage (150 nm mechanical xy resolution)
• Live Cell Incubation chamber (with temperature and C0₂ control)
• Spinning Disk Confocal
• Laser based Auto-focus
• Many Magnification options

Magnification	Immersion Media	Working Distance (mm)*	NA	Field of View, um2
2x-long	None	6.2	0.08	2.7-4 mm
10x-long	None	10	0.3	1351 x 1017
20x-long	None	6.6-7.8	0.45	675 x 509
40x-long	None	2.7-4	0.6	336 x 254
40x-high NA	None	0.29	0.95	338 x 254

• Designed to work with microscope slides, and nearly all plastic plate forms although glass bottomed (no 1.5 coverslip) low skirt multiwall plates work best.

Operetta Software: Harmony

• Designed for multi-user and multi-department environments
• Does not require computer programming or in depth knowledge of microscopy to operate
• Easy to use database of analytical features (texture analysis, cell tracing, emission quantitation, pattern recognition)
• Ready made solutions are easy to adapt to individual user needs
• Built in data management
• Data is stored as universally compatible OME TIFF files
• It is easy to streamline data migration to Columbus Database Server
• Volocity- 3D and 4D acquisition software is available for visualization and analysis.

Columbus Scope Server

• Independent image storage, management, and high performance analysis server system
• Images from any other microscope or high content screening system can be imported into Columbus for analysis
• Statistically significant, quantitative and multi-parametric data can be generated from input images
• Users have desktop access to data and analysis