Molecular Devices Nano High Content Screening
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Nano HCFS Platform
New in 2021, The Molecular Devices Nano High content screening system combines fully automated image collection (fluorescence and brightfield) with image analysis software to perform high throughput data analysis on cultured cells. The ‘microscope in a box’ platform is designed to be used with 96 or 384 well plates and uses a fully automated high-resolution stage to capture large fields of view (an entire well of a 384 well plate can be imaged at 4x) or smaller fields of view using fluorescence or transmitted light. High speed autofocus enables fast and consistent focusing across the plate. Live cells can be imaged with the environmental control chamber.
Available Magnifications (high NA Objectives):
| Magnification | Type | Immersion | NA | Working Distance (mm) |
| 4x | Plan Apo | AIR | 0.2 | 20 |
| 10x | Plan Fluar | AIR | 0.3 | 16 |
| 20x | Plan Fluor ELWD | AIR | 0.45 | 8.2-6.9 (CC) |
| 40x | Plan Fluor ELWD | AIR | 0.6 | 3.6-2.9 (CC) |
| 60x | Plan Fluor ELWD | AIR | 0.7 | 2.6-1.8 (CC) |
Fluorescence Filter Sets:
| Channel | Recommended Fluorophores | Excitation, nm | Dichroic, nm | Emission, nm |
| “Blue” | Dapi/Hoechst/AF405 | 377/50 (Violet) | 409 | 447/60 (Blue) |
| “Green” | GFP/FITC/AF488 | 474/27 (Blue) | 495 | 525/45 (Green) |
| “Red” | TRITC/RFP/DsRed/TdTomator/PI/Rhodamine/AF546/AF555 | 543/22 (Green) | 562 | 593/40 (Red) |
| “Red” | Texas Red/AF568/AF594/Cy3.5/Mitotracker Red | 562/40 (Green) | 593 | 624/40 (Red) |
| “Far Red” | Cy5/AF633/AF647/TO_PRO-3 | 628/40 (Red) | 660 | 692/40 (Far Red) |
Analysis Modules (using MetaXpress Image Analysis Software):
Count Nuclei Application Module, Multiwavelength Cell Scoring Module, Neurite Outgrowth:
Translocation, Transfluor, Live/Dead Application Module, Custom Module Editor