- Contact CMN staff to be trained on this equipment
Fluorescence Quantitation
About the GloMax®–Multi+ with Instinct™ Software
- Measures luminescence, fluorescence, visible or UV-visible absorbance in 6, 12, 24, 48, 96, and 384-well plate formats. Instinct™ software allows data analysis right on the touch screen.
- Single or dual auto injectors are available for flash-based luminescence applications or dual-reporter assays. Each injector system has a volume range of 25 – 200 μl in 5μl increments for 6- to 96-well plate formats.
- Luminescence module (factory installed):
>8 Logs Dynamic Range
Dual-masking System Reduces Crosstalk
3 x 10-21 Moles Luciferase Detection or 1 x 10-18 moles ATP
| Recommended injectors |
Application |
| 2 | Dual reporter assays |
| 0 | teady glo Luciferase assays |
| 1 | Flash glo luciferase assays |
| 1 | Cell viability/ATP assays |
| 1 | Kinetics |
Fluorescence Module (user installed):
To achieve high performance, the Fluorescence Module uses powerful light-emitting diodes (LEDs) as excitation sources. Five standard optical kits are available to measure the most popular fluorophores.
| Fluorescence Optical Kit |
Typical applications |
| UV Optical Kit Ex: 365 nm Em: 410 – 460 nm |
DNA Quantification (Hoechst dye), Enzyme Activity (4-methyl-umbelliferone) |
| Blue Optical Kit Ex: 490 nm Em: 510 – 570 nm |
DNA Quantitation, RNA Quantitation, Protein Labeling (Fluorescein), Protein Quantitation, Gene Expression (EGFP, rAcGFP) |
| Green Optical Kit Ex: 525 nm Em: 580 – 640 nm |
Nucleotide or Protein Labeling (Rhodamine, Cy3), Enzyme Activity (Rhodamine) |
| Red Optical Kit Ex: 625 nm Em: 660 – 720 nm |
Nucleotide or Protein Labeling (Cy5), RNA Quantitation |
| AFC Optical Kit Ex: 405 nm Em: 495 – 505 nm |
Cell Viability, Cytotoxicity, and Apoptosis Multiplex Assays |
Absorbance module (user installable):
The GloMax-Multi+ Microplate Multimode Reader provides you with two different options for making photometric measurements. A Visible Absorbance Module (450 – 750 nm) or a UV-Visible Absorbance Module (200 – 1100 nm). Both modules are user installable.
| Wavelength |
Applications |
| 260 | DNA and RNA Quantification |
| 280 | Protein |
| 450 | ELISA, QuantiCleave™ Protease Assay |
| 560 | BCA™ Protein Assays |
| 600 | Bradford Protein Assays, Coomassie Blue Protein Assays, PeroXOquant™ Quantitative Peroxide Assay |
| 750 | Lowry Protein Assay |
Contact the CMN staff to be trained on or if you have questions about this equipment.