Azure 500 Phosphorimaging System

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Fluorescence Quantitation

Illumination specifications:

Excitation			Emission
Illumination	Light Mode	Excitation Wavelength	Filter Name	Emission wavelength	Applications
Trans UV	UV Transilluminator	302 nm	Orange 595	595 nm ± 55 nm	Coomassie Blue, SYPRO Orange, SYBR Safe, SYBR Green, GFP, AF488
		365 nm
EPI Blue	LED-Blue	472 nm
EPI NIR	Laser	685 nm	IR 680	735 ± 28 nm	IR 680, Licor 700, AF680
	Laser	784 nm	IR 800	832 ± 37 nm	IR 800, Licor 800

Imaging modalities based on light source selection:

UV 302 transillumination: For dyes that are UV excitable (No EtBr please)

UV 365 transillumination: For cutting bands out of gel (No EtBr please)

EPI Blue: For dyes excitable in the “blue light” range (SYBR green)

Visible: For dyes that do not require additional excitation- will require Orange Tray

ChemiLuminescence: will require chemitray (black tray inside door)

Near Infrared (NIR): For Western blots labeled with NIR dyes: will require chemitray (black tray inside door)

Custom: You can modify the focus, exposure, light source, and use these in combination to create your own custom capture settings.