DNA libraries generated from 235 post mortem human brain samples were sequenced using 150 bp, paired end Illumina chemistry to a desired depth of 30X coverage. Reads were aligned to either the linear GRCh38 alt-free reference genome, the linear telomere to telomere hs1 reference genome, or the draft human pangenome.
Donna Werling
Donna Werling, PhD – Slide of the Week
Autism spectrum disorder (ASD) has a consistent 4:1 male prevalence, suggesting a role for sex-differential biology in risk.
Donna Werling, PhD – Slide of the Week
Autism spectrum disorder (ASD) is consistently diagnosed 3 to 5 times more frequently in males than females, a dramatically sex-biased prevalence that suggests the involvement of sex-differential biological factors in modulating risk. The genomic scale of transcriptomic analyses of human brain tissue can provide an unbiased approach for identifying genes and associated functional processes at the intersection of sex-differential and ASD-impacted neurobiology.
Donna Werling, PhD – Slide of the Week
Gene expression levels vary across developmental stage, cell type, and region in the brain. Genomic variants also contribute to the variation in expression, and some neuropsychiatric disorder loci may exert their effects through this mechanism. To investigate these relationships, we present BrainVar, a unique resource of paired whole-genome and bulk tissue RNA sequencing from the dorsolateral prefrontal cortex of 176 individuals across prenatal and post- natal development.