Advancing knowledge of human development, developmental disabilities, and neurodegenerative diseases.
Neuroinflammation plays an important role in ischemic brain injury and recovery, however the interplay between brain development and the neuroinflammatory response is poorly understood. We previously described age-dependent differences in the microglial response and the effect of microglial inhibition.
Immunostaining and microglia isolation via magnetic cell sorting was performed at postnatal day 2, 9, 30 and 60 (P2, P9, P30, P60). Microglia branching and soma size were quantified using semiautomated software analysis (Imaris, Oxford Insturments).
One Wednesday in September, 15-year-old Tristan Thurman went to soccer practice at his high school. The team had just lost a game the day before because the other team had scored goals heading the ball, so this practice session was dedicated to heading drills. Tristan remembers feeling slightly dizzy after practice. By that Friday morning, …
Title: Suppression of microglia activation after hypoxia-ischemia results in age-dependent improvements in neurologic injury. Legend: Figure 1. Minocycline treatment suppresses the microglial response to hypoxia-ischemia (HI) in neonatal and juvenile mice. The number of CD11b+/CD45+ cells in ipsilateral hippocampus (A) cortex (B), and striatum (C) relative to the corresponding contralateral brain region (IL/CL) were determined …
Brain injuries caused by a lack of oxygen and blood flow affect many thousands of children every year, yet “we still don’t have effective treatments for them,” says Waisman researcher Peter Ferrazzano, MD.
Title: Suppression of microglia activation after hypoxia-ischemia results in age-dependent improvements in neurologic injury. Legend: P9 and P30 mice were exposed to unilateral hypoxia-ischemia and treated with minocycline or vehicle. A, B. Double immunohistological staining for Iba1 (green) and MAP2 (red) proteins in the CA1 region of the hippocampus at 2 days post-HI is shown. …
