Advancing knowledge of human development, developmental disabilities, and neurodegenerative diseases.
Title: Cell cycle-linked MeCP2 phosphorylation modulates adult neurogenesis Legend: Representative images (left) of adult neural progenitor cells (aNPCs) isolated from wild type (WT) and Mecp2S421A;S424A/y hippocampus with BrdU pulse labeling, followed by immunocytochemistry analysis. (middle) Representative images of Tuj1+ neurons differentiated from WT and Mecp2S421A;S424A/y aNPCs. (right) Representative images of GFAP+ astrocyte differentiated from WT and …
Title: Up-regulation of NHE1 protein expression in reactive hippocampal astrocytes Legend: A.Expression of NHE1 protein in hippocampal astrocytes. Immunofluorescence signals of NHE1 and GFAP were shown under normoxia control or OGD (2 h) plus 0 h, 5 h, or 24 h REOX. Green: GFAP, red: NHE1 (arrows), blue: To-pro-3 nuclear staining. Scale Bar: 20 mm. …
Title: iPSC-Derived Forebrain Neurons from FXS Individuals Show Defects in Initial Neurite Outgrowth Legend: Left panel: Skin cells from individuals with fragile X syndrome (FXS) can be reprogrammed into induced pluripotent stem cells (iPSCs) that have characteristics of human embryonic stem cells. These iPSCs can then be differentiated into forebrain cortical neurons to study neuronal …
Title: Insulin resistance predicts brain amyloid deposition in late middle-aged adults Legend: Healthy late middle-aged adults with higher insulin resistance (based on fasting glucose and insulin levels) show greater amyloid deposition on [C11]PIB-PET imaging. The results suggest that metabolic dysfunction in midlife may contribute to Alzheimer’s pathology. Shown on the left for illustrative purposes are …
Title: Sequencing Library Generation Legend: Each probe contains two parts of sequences: one for targeting interested CFTR gene regions, and another for annealing to PCR primers. The DNA templates containing targeted CFTR regions were generated by a sequential process consistent with hybridization, extension, and ligation. The indexes included in PCR primers allow multiplexing of 48 individual DNA …
Title: Sensory subtypes in children with autism using a latent profile transition analysis Legend: Four clinically distinct and homogeneous sensory subtypes were found to characterize children with autism spectrum disorder (ASD) in a large longitudinal national sample and also shown to be stable (91%) over one year. The Mild Subtype (N = 308, 29%) described …
Titel: Imaging of TFG, a protein implicated in neurodegenerative disease, across a variety of scales Legend: Staining of TFG (green) and a marker of the endoplasmic reticulum (red) in human epithelial cells (left). The localization of TFG was further defined by immunogold electron microscopy (15 nm gold particles), highlighting its accumulation among ER-derived transport carriers …
Title: Classroom observations of social interactions between high school students with significant disabilities and their general education peers Legend: The average rate (number) of interactions per hour for focus students with significant disabilities with any general education classmate across peer support and comparison groups during pre (no intervention for both groups) and post (peer support intervention; …
Title: Multiple contrast MRI with retrospective motion correction Legend: Representative example of multiple image contrasts from a new brain imaging technique MPnRAGE developed in the Alexander Lab in the Waisman Brain Imaging Core. The MPnRAGE method can generate multiple inversion recovery contrasts, quantitative T1 relaxometry, and tissue specific (gray matter or white matter) maps. Whole …
