May is #ALSAwarenessMonth and Waisman investigator Su-Chun Zhang, MD, PhD, uses stem cells to uncover the cause of ALS with the hope of developing treatments and therapies.
iPSC
New gene editing tool driving stem cell services and discovery
A new gene editing service provides researchers on campus with genetically engineered pluripotent stem cell lines derived using CRISPR-Cas9. The UW-Madison iPSC Reprogramming and Human Stem Cell Gene Editing Service is co-operated by the Waisman …
Randy Ashton, PhD – Slide of the Week
Transplantation of human pluripotent stem cell (hPSC)-derived neurons into chick embryos is an established preliminary assay to evaluate engraftment potential. Yet, with recent advances in deriving diverse human neuronal subtypes, optimizing and standardizing such transplantation methodology for specific subtypes at their correlated anatomical sites is still required.
Masatoshi Suzuki, DVM, PhD – Slide of the Week
Human induced-pluripotent stem cells are a promising resource for propagation of myogenic progenitors. Our group recently reported a unique protocol for the derivation of myogenic progenitors directly (without genetic modification) from human pluripotent cells using free-floating spherical culture.
Anita Bhattacharyya, PhD – Slide of the Week
Generation of Human Induced Pluripotent Stem Cells from Unaffected Neonatal Skin Cells – We have generated and characterized iPSC clones from three unaffected, neonatal individuals using non-integrating episomal reprogramming plasmids expressing OCT4, SOX2, LIN28, KLF4, and c-Myc.
Randolph Ashton, PhD
Human pluripotent stem cell-derived neural organoids provide unprecedented potential to recapitulate human brain and spinal cord tissues in vitro. However, organoid morphogenesis relies upon cell-intrinsic self-assembly of biomimetic tissue structures in the absence of normal developmental constraints and morphogen signaling centers.
A decade after stem cell feat, research ramps up
A decade after scientists announced the development of induced pluripotent stem cells, Waisman investigators, including Su-Chun Zhang and David Gamm, continue to use these cells to research and develop potential therapies for several disorders and conditions, such as ALS, Parkinson’s disease, spinal cord injury, and macular degeneration.
“Assembling Tridimensional Brain Models to Study Human Development and Disease”
Sergiu P. Pasca, MD – Stanford University Pasca’s lab is interested in deciphering the molecular and cellular mechanisms of neuropsychiatric disorders. To achieve this, they employ a multidisciplinary approach involving human genetics, molecular and developmental …
Anita Bhattacharyya, PhD
Title: Creation of a human reporter stem cell line for detecting fragile X mental retardation (FMR1) gene reactivation in human neural cells. Legend: Fragile X syndrome (FXS) is the most common inherited cause of intellectual …
Derek Hei, PhD
Title: iPSC derived Optic Vesicles (OVs)/Neural Retinal Progenitors (NRPs) – Production, Characterization and Cryopreservation Legend: 1) Representative pictures of selected OVs derived from iPSCs grown on E8/Matrigel. iPSCs, cultured on E8/Matrigel, mTeSR1/Matrigel, and E8/vitronectin, were …