Generation of Human Induced Pluripotent Stem Cells from Unaffected Neonatal Skin Cells – We have generated and characterized iPSC clones from three unaffected, neonatal individuals using non-integrating episomal reprogramming plasmids expressing OCT4, SOX2, LIN28, KLF4, and c-Myc.
iPSC
Randolph Ashton, PhD
Human pluripotent stem cell-derived neural organoids provide unprecedented potential to recapitulate human brain and spinal cord tissues in vitro. However, organoid morphogenesis relies upon cell-intrinsic self-assembly of biomimetic tissue structures in the absence of normal developmental constraints and morphogen signaling centers.
A decade after stem cell feat, research ramps up
A decade after scientists announced the development of induced pluripotent stem cells, Waisman investigators, including Su-Chun Zhang and David Gamm, continue to use these cells to research and develop potential therapies for several disorders and conditions, such as ALS, Parkinson’s disease, spinal cord injury, and macular degeneration.
“Assembling Tridimensional Brain Models to Study Human Development and Disease”
Sergiu P. Pasca, MD – Stanford University Pasca’s lab is interested in deciphering the molecular and cellular mechanisms of neuropsychiatric disorders. To achieve this, they employ a multidisciplinary approach involving human genetics, molecular and developmental …
Anita Bhattacharyya, PhD
Title: Creation of a human reporter stem cell line for detecting fragile X mental retardation (FMR1) gene reactivation in human neural cells. Legend: Fragile X syndrome (FXS) is the most common inherited cause of intellectual …
Derek Hei, PhD
Title: iPSC derived Optic Vesicles (OVs)/Neural Retinal Progenitors (NRPs) – Production, Characterization and Cryopreservation Legend: 1) Representative pictures of selected OVs derived from iPSCs grown on E8/Matrigel. iPSCs, cultured on E8/Matrigel, mTeSR1/Matrigel, and E8/vitronectin, were …
UW Eye Researchers Publish Second Study of Stem Cells and Blinding Eye Disease
A team of eye researchers at the University of Wisconsin-Madison have used a custom stem cell model of a rare but blinding eye disease to test whether a commonly used drug might offer hope for treatment.
Anita Bhattacharyya, PhD
Title: iPSC-Derived Forebrain Neurons from FXS Individuals Show Defects in Initial Neurite Outgrowth Legend: Left panel: Skin cells from individuals with fragile X syndrome (FXS) can be reprogrammed into induced pluripotent stem cells (iPSCs) that …
Vision Research Month
June is Vision Research Awareness Month and the Waisman Center is home to a comprehensive vision research program led by David Gamm, MD, PhD. Gamm, a Waisman Center investigator, associate professor of ophthalmology and visual sciences, and Director of the McPherson Eye Research Institute, studies human retinal development and potential therapies for retinal degenerative diseases.
Hearing research at the Waisman Center
More than 278 million people are affected by hearing loss caused by damaged hair cells in the inner ear that process sound to the brain. Waisman Center scientists work to research novel treatments and therapies for individuals with hearing loss that include stem cell research and cochlear implants.